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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Epithelial Cell Adhesion Molecule Regulation Is Associated with the Maintenance of the Undifferentiated Phenotype of Human Embryonic Stem Cells
doi: 10.1074/jbc.M109.077081
Figure Lengend Snippet: Methylation status of EpCAM promoter regions in undifferentiated and differentiated hESCs. A, schematic representation of the EpCAM gene promoter region. Primers for MSP and bisulfite sequencing used in the study are indicated. B, MSP analysis of the EpCAM gene promoter region in undifferentiated and differentiated H9 cells. The PCR products that were methylated (M) were generated by methylation-specific primers, and those that were unmethylated (U) were generated by primers specific for unmethylated DNA. C, mapping the methylation status of the CpG islands in the promoter region of the EpCAM gene by bisulfite sequencing. Each row of squares represents a single plasmid cloned and sequenced from PCR products generated from amplification of bisulfite-treated DNA. Open squares, unmethylated cytosines; filled squares, methylated cytosines. Most CpGs in the promoter region in both undifferentiated and differentiated H9 cells were unmethylated.
Article Snippet: Completely methylated and
Techniques: Methylation, Methylation Sequencing, Generated, Plasmid Preparation, Clone Assay, Amplification